BK beta4 ECFP
(Plasmid
#113564)
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Purposehuman BK beta4 subunit in C-term translational frame with ECFP (ECFP-N1)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneECFP-N1
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Backbone manufacturerClontech
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Modifications to backboneIdentical to beta4-EGFP plasmid (Addgene #113560) but with ECFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKcnmb4
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Alt namebeta4
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SpeciesH. sapiens (human)
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Insert Size (bp)748
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Entrez GeneKCNMB4
- Promoter CMV
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Tag
/ Fusion Protein
- ECFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BK beta4 ECFP was a gift from Robert Brenner (Addgene plasmid # 113564 ; http://n2t.net/addgene:113564 ; RRID:Addgene_113564)