TfRΔEctoR1-eGFP A206K
(Plasmid
#113554)
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PurposeTransmembrane model receptor to probe receptor internalization via clathrin mediated pathway
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfRΔEctoR1-eGFP A206K
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MutationeGFP A206K
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TfRΔEctoR1-eGFP A206K was a gift from Jeanne Stachowiak (Addgene plasmid # 113554 ; http://n2t.net/addgene:113554 ; RRID:Addgene_113554) -
For your References section:
Entropic Control of Receptor Recycling Using Engineered Ligands. DeGroot ACM, Busch DJ, Hayden CC, Mihelic SA, Alpar AT, Behar M, Stachowiak JC. Biophys J. 2018 Mar 27;114(6):1377-1388. doi: 10.1016/j.bpj.2018.01.036. 10.1016/j.bpj.2018.01.036 PubMed 29590595
