pRSET his-mRFP
(Plasmid
#113553)
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PurposeFor bacterial expression and purification of mRFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehis-mRFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET his-mRFP was a gift from Jeanne Stachowiak (Addgene plasmid # 113553 ; http://n2t.net/addgene:113553 ; RRID:Addgene_113553) -
For your References section:
Entropic Control of Receptor Recycling Using Engineered Ligands. DeGroot ACM, Busch DJ, Hayden CC, Mihelic SA, Alpar AT, Behar M, Stachowiak JC. Biophys J. 2018 Mar 27;114(6):1377-1388. doi: 10.1016/j.bpj.2018.01.036. 10.1016/j.bpj.2018.01.036 PubMed 29590595