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PurposeFor bacterial expression and purification of eGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehis-eGFP
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MutationA206K
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET his-eGFP was a gift from Jeanne Stachowiak (Addgene plasmid # 113551 ; http://n2t.net/addgene:113551 ; RRID:Addgene_113551) -
For your References section:
Intrinsically disordered proteins drive membrane curvature. Busch DJ, Houser JR, Hayden CC, Sherman MB, Lafer EM, Stachowiak JC. Nat Commun. 2015 Jul 24;6:7875. doi: 10.1038/ncomms8875. 10.1038/ncomms8875 PubMed 26204806