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pcDNA5/FRT/TO-Intron-CterFlag-MePCE
(Plasmid #113549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO/intron
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5344
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MePCE
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2067
  • Entrez Gene
    MEPCE (a.k.a. BCDIN3)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-Intron-CterFlag-MePCE was a gift from Blerta Xhemalce (Addgene plasmid # 113549 ; http://n2t.net/addgene:113549 ; RRID:Addgene_113549)

  • For your References section:

    Crosstalk between the RNA Methylation and Histone-Binding Activities of MePCE Regulates P-TEFb Activation on Chromatin. Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M, Xhemalce B. Cell Rep. 2018 Feb 6;22(6):1374-1383. doi: 10.1016/j.celrep.2018.01.028. 10.1016/j.celrep.2018.01.028 PubMed 29425494
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