pGEX-5X-1-LARP7
(Plasmid
#113545)
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PurposeProduces an N-terminal GST-tagged full length LARP7 protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-5X-1
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Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4972
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLARP7
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SpeciesH. sapiens (human)
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Insert Size (bp)1746
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Entrez GeneLARP7 (a.k.a. ALAZS, HDCMA18P, PIP7S, hLARP7)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-5X-1-LARP7 was a gift from Blerta Xhemalce (Addgene plasmid # 113545 ; http://n2t.net/addgene:113545 ; RRID:Addgene_113545) -
For your References section:
Crosstalk between the RNA Methylation and Histone-Binding Activities of MePCE Regulates P-TEFb Activation on Chromatin. Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M, Xhemalce B. Cell Rep. 2018 Feb 6;22(6):1374-1383. doi: 10.1016/j.celrep.2018.01.028. 10.1016/j.celrep.2018.01.028 PubMed 29425494