pGEX-4T-1 UBXN2A
(Plasmid
#113505)
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PurposeBacterial expression of GST-tagged UBXN2A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUBXN2A
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Alt nameUBXD4
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SpeciesH. sapiens (human)
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Insert Size (bp)780
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Entrez GeneUBXN2A (a.k.a. UBXD4)
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Tag
/ Fusion Protein
- GST-Thrombin cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1 UBXN2A was a gift from Hemmo Meyer (Addgene plasmid # 113505 ; http://n2t.net/addgene:113505 ; RRID:Addgene_113505) -
For your References section:
Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation. Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H. Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020. 10.1016/j.molcel.2018.09.020 PubMed 30344098