pGEX-6P-1 p37 SHPmut
(Plasmid #113503)

• Purpose: Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box
• Depositing Lab: Hemmo Meyer
• Publication: Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020.

Backbone

• Vector backbone: pGEX-6P-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: p37
• Alt name: UBXN2B
• Species: M. musculus (mouse)
• Insert Size (bp): 996
• Mutation: F215A/E218A/QKL220-222AAA
• Entrez Gene: Ubxn2b (a.k.a. 3110003A22Rik, 6430407D20Rik, AI451665, p37)
• Tag / Fusion Protein:
  • GST-PreScission protease site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)

Resource Information

• Supplemental Documents:
  • pGEX-6P-1_p37 SHPmut.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry