pEGFP-UBE4B
(Plasmid #113499)

• Purpose: Expression of human UBE4B with N-terminal GFP tag
• Depositing Lab: Hemmo Meyer
• Publication: Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 10.1074/mcp.RA117.000471.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBE4B
• Alt name: UFD2
• Alt name: HDNB1
• Species: H. sapiens (human)
• Insert Size (bp): 3631
• Entrez Gene: UBE4B (a.k.a. E4, HDNB1, UBOX3, UFD2, UFD2A)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry