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pcDNA5FRT/TO-SVIP-Strep-HA
(Plasmid #113491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT-TO-cSH-GW
  • Backbone manufacturer
    Ruedi Aebersold
  • Backbone size w/o insert (bp) 7017
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SVIP
  • Alt name
    Small VCP/p97-interacting protein
  • Species
    H. sapiens (human)
  • Mutation
    wildtype without stop codon
  • Entrez Gene
    SVIP
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xstrep-HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT/TO-SVIP-Strep-HA was a gift from Hemmo Meyer (Addgene plasmid # 113491 ; http://n2t.net/addgene:113491 ; RRID:Addgene_113491)

  • For your References section:

    AP-SWATH reveals direct involvement of VCP/p97 in integrated stress response signaling through facilitating CReP/PPP1R15B degradation. Hulsmann J, Kravic B, Weith M, Gstaiger M, Aebersold RH, Collins BC, Meyer H. Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 10.1074/mcp.RA117.000471. 10.1074/mcp.RA117.000471 PubMed 29599191
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