pcDNA5FRT/TO-PNGase-Strep-HA
(Plasmid #113490)

• Purpose: Expression of human PNGase with C-terminal strep-HA tag
• Depositing Lab: Hemmo Meyer
• Publication: Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 10.1074/mcp.RA117.000471.

Backbone

• Vector backbone: pcDNA5-FRT-TO-cSH-GW
• Backbone manufacturer: Ruedi Aebersold
• Backbone size w/o insert (bp): 7017
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PNGase
• Alt name: NGLY1
• Alt name: PNG1
• Species: H. sapiens (human)
• Mutation: wildtype without stop codon
• Entrez Gene: NGLY1 (a.k.a. CDDG, CDG1V, PNG-1, PNG1, PNGase)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2xstrep-HA (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry