pcDNA5FRT/TO-DVC1-Strep-HA
(Plasmid
#113481)
-
PurposeExpression of human DVC1 with C-terminal strep-HA tag
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 113481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA5-FRT-TO-cSH-GW
-
Backbone manufacturerRuedi Aebersold
- Backbone size w/o insert (bp) 7017
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDVC1
-
Alt nameSPRTN
-
Alt nameC1orf124
-
SpeciesH. sapiens (human)
-
Mutationwildtype without stop codon
-
Entrez GeneSPRTN (a.k.a. C1orf124, DVC1, PRO4323, spartan)
- Promoter CMV
-
Tag
/ Fusion Protein
- 2xstrep-HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5FRT/TO-DVC1-Strep-HA was a gift from Hemmo Meyer (Addgene plasmid # 113481 ; http://n2t.net/addgene:113481 ; RRID:Addgene_113481) -
For your References section:
AP-SWATH reveals direct involvement of VCP/p97 in integrated stress response signaling through facilitating CReP/PPP1R15B degradation. Hulsmann J, Kravic B, Weith M, Gstaiger M, Aebersold RH, Collins BC, Meyer H. Mol Cell Proteomics. 2018 Mar 29. pii: RA117.000471. doi: 10.1074/mcp.RA117.000471. 10.1074/mcp.RA117.000471 PubMed 29599191