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Addgene

pCMV-hA3F-BE3
(Plasmid #113414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Backbone size w/o insert (bp) 3399
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hA3F-BE3
  • Alt name
    pCMV-hAPOBEC3F-XTEN-D10A-SGGS-UGI-SGGS-NLS
  • Species
    H. sapiens (human); S. pyogenes and Bacteriophage PBS2
  • Insert Size (bp)
    5565
  • Entrez Gene
    APOBEC3F (a.k.a. A3F, ARP8, BK150C2.4.MRNA, KA6)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH poly reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-hA3F-BE3 was a gift from Jia Chen (Addgene plasmid # 113414 ; http://n2t.net/addgene:113414 ; RRID:Addgene_113414)

  • For your References section:

    Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. 10.1038/nbt.4198 PubMed 30125268
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