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Addgene

pGC4780
(Plasmid #113408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGC4750
  • Backbone size w/o insert (bp) 5222
  • Total vector size (bp) 5333
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    158_33121123_1
  • Species
    Synthetic
  • Insert Size (bp)
    111
  • Tag / Fusion Protein
    • fused to codon deptimized sfGFP with Flexible linker and TEV cleavage site (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CATGCACATAAGGAGGTACCATAATG
  • 3′ sequencing primer ACCCGCCCGATCCACCGGATCCACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGC4780 was a gift from Adam Arkin & Guillaume Cambray (Addgene plasmid # 113408 ; http://n2t.net/addgene:113408 ; RRID:Addgene_113408)

  • For your References section:

    Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli. Cambray G, Guimaraes JC, Arkin AP. Nat Biotechnol. 2018 Nov;36(10):1005-1015. doi: 10.1038/nbt.4238. Epub 2018 Sep 24. 10.1038/nbt.4238 PubMed 30247489
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