pGC4780
(Plasmid #113408)

• Purpose: Translation reporter with inducible translation coupling (high native GFP expression)
• Depositing Labs: Adam Arkin, Guillaume Cambray
• Publication: Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-1015. doi: 10.1038/nbt.4238. Epub 2018 Sep 24.

Backbone

• Vector backbone: pGC4750
• Backbone size w/o insert (bp): 5222
• Total vector size (bp): 5333
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 158_33121123_1
• Species: Synthetic
• Insert Size (bp): 111
• Tag / Fusion Protein:
  • fused to codon deptimized sfGFP with Flexible linker and TEV cleavage site (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: kpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CATGCACATAAGGAGGTACCATAATG
• 3′ sequencing primer: ACCCGCCCGATCCACCGGATCCACC

Resource Information

• Supplemental Documents:
  • pGC4780.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry