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Addgene

a4 ecto pcDNA3.1 hygro(-)
(Plasmid #113406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 hygro(-)
  • Backbone manufacturer
    Thermofisher
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 8700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human alpha 4 ectodomain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3091
  • Promoter CMV
  • Tag / Fusion Protein
    • Strep II tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    a4 ecto pcDNA3.1 hygro(-) was a gift from Timothy Springer (Addgene plasmid # 113406 ; http://n2t.net/addgene:113406 ; RRID:Addgene_113406)

  • For your References section:

    Energy landscape differences among integrins establish the framework for understanding activation. Li J, Springer TA. J Cell Biol. 2018 Jan 2;217(1):397-412. doi: 10.1083/jcb.201701169. Epub 2017 Nov 9. 10.1083/jcb.201701169 PubMed 29122968
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