pNB3033
(Plasmid
#113397)
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PurposeTy3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29a
- Total vector size (bp) 6108
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTy3 Gag3 codon optimized for E. coli expression
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Alt nameTy3 Gag3
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- Gag3-6XHis tag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNB3033 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113397 ; http://n2t.net/addgene:113397 ; RRID:Addgene_113397)