pNB2176
(Plasmid
#113395)
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PurposeTy3-IN-GFP; alpha factor inducible
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYES2.0
- Total vector size (bp) 10862
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTy3 with integrase-green fluorescent protein
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Alt nameTy3 IN-GFP
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- IN-GFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNB2176 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113395 ; http://n2t.net/addgene:113395 ; RRID:Addgene_113395) -
For your References section:
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components. Beliakova-Bethell N, Beckham C, Giddings TH Jr, Winey M, Parker R, Sandmeyer S. RNA. 2006 Jan;12(1):94-101. doi: 10.1261/rna.2264806. 10.1261/rna.2264806 PubMed 16373495
