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Addgene

pNB2629
(Plasmid #113394)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113394 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS315
  • Total vector size (bp) 7620
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MS2 bacteriophage coat protein
  • Alt name
    MS2
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • red fluorescent protein (RFP), codon-optimized for S. cerevisiae (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNB2629 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113394 ; http://n2t.net/addgene:113394 ; RRID:Addgene_113394)
  • For your References section:

    Sequence requirements for localization and packaging of Ty3 retroelement RNA. Clemens K, Bilanchone V, Beliakova-Bethell N, Larsen LS, Nguyen K, Sandmeyer S. Virus Res. 2013 Feb;171(2):319-31. doi: 10.1016/j.virusres.2012.10.008. Epub 2012 Oct 13. 10.1016/j.virusres.2012.10.008 PubMed 23073180