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Addgene

pTD3685
(Plasmid #113391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Total vector size (bp) 8859
  • Modifications to backbone
    multiple cloning region was modified
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ty3
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The two Ty3 ORFs are separated by a frameshift, and a translational frameshift event results in expression of a single fusion protein from both ORFs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTD3685 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113391 ; http://n2t.net/addgene:113391 ; RRID:Addgene_113391)

  • For your References section:

    Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K, Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S. PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 10.1371/journal.pgen.1005528. eCollection 2015. PGENETICS-D-15-00916 [pii] PubMed 26421679
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