pVB3011
(Plasmid
#113389)
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PurposeGalactose-inducible L-BC gag: cure killer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYES2.0
- Total vector size (bp) 7954
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameL-BC Gag
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1986
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MutationPlease see depositor comments
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Plasmid contains a truncated L-BC gag which is sufficient for curing of L-BC killer
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVB3011 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113389 ; http://n2t.net/addgene:113389 ; RRID:Addgene_113389) -
For your References section:
Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K, Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S. PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 10.1371/journal.pgen.1005528. eCollection 2015. PGENETICS-D-15-00916 [pii] PubMed 26421679