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human b1 ecto-pIRES
(Plasmid #113387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 8465
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human beta 1 ectodomain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2365
  • Promoter CMV
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer custom
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human b1 ecto-pIRES was a gift from Timothy Springer (Addgene plasmid # 113387 ; http://n2t.net/addgene:113387 ; RRID:Addgene_113387)

  • For your References section:

    Conformational equilibria and intrinsic affinities define integrin activation. Li J, Su Y, Xia W, Qin Y, Humphries MJ, Vestweber D, Cabanas C, Lu C, Springer TA. EMBO J. 2017 Mar 1;36(5):629-645. doi: 10.15252/embj.201695803. Epub 2017 Jan 25. 10.15252/embj.201695803 PubMed 28122868
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