a5-ecto-pcDNA31hygro
(Plasmid #113386)

• Purpose: Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp
• Depositing Lab: Timothy Springer
• Publication: Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 10.15252/embj.201695803. Epub 2017 Jan 25.

Backbone

• Vector backbone: pcDNA3.1 hygro(-)
• Backbone manufacturer: Thermofisher
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 8800
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human alpha 5 ectodomain
• Species: H. sapiens (human)
• Insert Size (bp): 3157
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • Strep II tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry