ATP9A-luciferase
(Plasmid
#113354)
-
Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 113354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL4-TK
-
Backbone manufacturerPromega
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameATP9A-enhancer
-
SpeciesH. sapiens (human)
-
Entrez GeneATP9A (a.k.a. ATPIIA, NEDGBA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ATP9A-luciferase was a gift from Ralf Jauch (Addgene plasmid # 113354 ; http://n2t.net/addgene:113354 ; RRID:Addgene_113354) -
For your References section:
DNA-mediated dimerization on a compact sequence signature controls enhancer engagement and regulation by FOXA1. Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, Cojocaru V, Prabhakar S, Jauch R. Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 10.1093/nar/gky259. 10.1093/nar/gky259 PubMed 29669022