pJY3948
(Plasmid
#113338)
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Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJY3872
- Total vector size (bp) 7894
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name2-pyrone synthase, codon optimized for Y. lipolytica
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Alt name2-PS
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SpeciesGerbera hybrida
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Insert Size (bp)1239
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MutationPlease see depositor comments
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Tag
/ Fusion Protein
- 6XHis (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Plasmid contains a G296C mutation in 2-pyrone synthase. This mutation is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJY3948 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113338 ; http://n2t.net/addgene:113338 ; RRID:Addgene_113338) -
For your References section:
Bioengineering triacetic acid lactone production in Yarrowia lipolytica for pogostone synthesis. Yu J, Landberg J, Shavarebi F, Bilanchone V, Okerlund A, Wanninayake U, Zhao L, Kraus G, Sandmeyer S. Biotechnol Bioeng. 2018 May 19. doi: 10.1002/bit.26733. 10.1002/bit.26733 PubMed 29777591
