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Addgene

pKP4010
(Plasmid #113336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKP3915
  • Total vector size (bp) 11641
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Artificial Transcription Factor (McIsaac et al, 2013)
  • Alt name
    ATF
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1485

Gene/Insert 2

  • Gene/Insert name
    Ty3 Integrase
  • Alt name
    IN
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1722
  • Tag / Fusion Protein
    • 3X-FLAG/8X-Gly linker (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP4010 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113336 ; http://n2t.net/addgene:113336 ; RRID:Addgene_113336)

  • For your References section:

    Local features determine Ty3 targeting frequency at RNA polymerase III transcription start sites. Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, Bilanchone V, Sandmeyer SB. Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. Epub 2019 Jun 27. 10.1101/gr.240861.118 PubMed 31249062
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