pMT3928
(Plasmid
#113331)
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Purposecontrol for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT3923
- Total vector size (bp) 10508
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Vector typeUnspecified
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Selectable markersLEU2, URA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namebeta-isopropyl malate dehydrogenase
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Alt nameLEU2
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SpeciesYarrowia lipolytica
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Insert Size (bp)2087
Gene/Insert 2
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Gene/Insert namecodon-optimized Hermes transposase
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SpeciesYarrowia lipolytica
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Insert Size (bp)1863
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT3928 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113331 ; http://n2t.net/addgene:113331 ; RRID:Addgene_113331) -
For your References section:
Functional genomics for the oleaginous yeast Yarrowia lipolytica. Patterson K, Yu J, Landberg J, Chang I, Shavarebi F, Bilanchone V, Sandmeyer S. Metab Eng. 2018 May 22;48:184-196. doi: 10.1016/j.ymben.2018.05.008. 10.1016/j.ymben.2018.05.008 PubMed 29792930
