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Addgene

pKJ-1
(Plasmid #11333)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pgk-1 5' and 3' regions
  • Alt name
    phosphoglycerate kinase-1
  • Alt name
    PGKneo
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Pgk1 (a.k.a. Pgk-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes 500 bp mouse PGK promoter, 832 bp neo, 460 bp PGK 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKJ-1 was a gift from Michael McBurney (Addgene plasmid # 11333 ; http://n2t.net/addgene:11333 ; RRID:Addgene_11333)

  • For your References section:

    Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Adra CN, Boer PH, McBurney MW. Gene. 1987 . 60(1):65-74. 10.1016/0378-1119(87)90214-9 PubMed 3440520
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