pRL-TK let7 I
(Plasmid #11332)

• Depositing Lab: Phil Sharp
• Publication: Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Backbone

• Vector backbone: pRL-TK
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4045
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2 binding sites for let-7a miRNA
• Mutation: Mutation I from figure 4A of paper.
• Tag / Fusion Protein:
  • Rr-luc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See comment (unknown if destroyed)
• 3′ cloning site: See comment (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.

Mutant binding site sequence is - TTACTTCA