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Addgene

pTP284
(Plasmid #113312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nbs1
  • Species
    H. sapiens (human)
  • Entrez Gene
    NBN (a.k.a. AT-V1, AT-V2, ATV, NBS, NBS1, P95)
  • Promoter polyhedrin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that 2 mutations in Gentamicin (C4S and K98R) were found during Addgene's quality control process. The depositing lab has noted that these 2 mutations do not affect Gentamicin selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP284 was a gift from Tanya Paull (Addgene plasmid # 113312 ; http://n2t.net/addgene:113312 ; RRID:Addgene_113312)

  • For your References section:

    Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair. Myler LR, Gallardo IF, Soniat MM, Deshpande RA, Gonzalez XB, Kim Y, Paull TT, Finkelstein IJ. Mol Cell. 2017 Sep 7;67(5):891-898.e4. doi: 10.1016/j.molcel.2017.08.002. Epub 2017 Aug 31. 10.1016/j.molcel.2017.08.002 PubMed 28867292
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