pAW091
(Plasmid
#113253)
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PurposepUC19 backbone with S. pyogenes CRISPR array
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 2845
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Vector typeIntegration substrate
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS. pyogenes CRISPR array
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SpeciesS. pyogenes
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Insert Size (bp)569
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GenBank IDNC_002737.2
- Promoter NA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site AflIII (unknown if destroyed)
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAW091 was a gift from Jennifer Doudna (Addgene plasmid # 113253 ; http://n2t.net/addgene:113253 ; RRID:Addgene_113253) -
For your References section:
Protecting genome integrity during CRISPR immune adaptation. Wright AV, Doudna JA. Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 10.1038/nsmb.3289. Epub 2016 Sep 5. 10.1038/nsmb.3289 PubMed 27595346