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Addgene

pAW092
(Plasmid #113250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16bMBP
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7734
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    S. pyogenes Cas1
  • Alt name
    SpyCas1
  • Species
    S. pyogenes
  • Insert Size (bp)
    870
  • Mutation
    H205A
  • GenBank ID
    NC_002737.2
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CAGCGGTCGTCAGACTGTCG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAW092 was a gift from Jennifer Doudna (Addgene plasmid # 113250 ; http://n2t.net/addgene:113250 ; RRID:Addgene_113250)

  • For your References section:

    Protecting genome integrity during CRISPR immune adaptation. Wright AV, Doudna JA. Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 10.1038/nsmb.3289. Epub 2016 Sep 5. 10.1038/nsmb.3289 PubMed 27595346
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