pAW206
(Plasmid #113246)

• Purpose: E. coli expression vector for IHFa K15A + IHFb
• Depositing Lab: Jennifer Doudna
• Publication: Wright et al Science. 2017 Sep 15;357(6356):1113-1118. doi: 10.1126/science.aao0679. Epub 2017 Jul 20.

Backbone

• Vector backbone: pACYC
• Backbone size w/o insert (bp): 3600
• Total vector size (bp): 4382
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: IHFa K15A
• Species: E. coli
• Insert Size (bp): 297
• Mutation: K15A
• Entrez Gene: ihfA (a.k.a. b1712, ECK1710, hid, himA)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGATCTCGACGCTCTCCCT
• 3′ sequencing primer: GATTATGCGGCCGTGTACAA

Gene/Insert 2

• Gene/Insert name: IHFb
• Species: E. coli
• Insert Size (bp): 282
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTGTACACGGCCGCATAATC
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pAW206.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry