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Addgene

pAW218
(Plasmid #113240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF1b
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4909
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas1
  • Species
    E. coli
  • Insert Size (bp)
    924

Gene/Insert 2

  • Gene/Insert name
    Cas2
  • Species
    E. coli
  • Insert Size (bp)
    285

Gene/Insert 3

  • Gene/Insert name
    Leader-repeat
  • Species
    E. coli
  • Insert Size (bp)
    154
  • Mutation
    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTAATTAATGATTTTTT (IHF binding site flip)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAW218 was a gift from Jennifer Doudna (Addgene plasmid # 113240 ; http://n2t.net/addgene:113240 ; RRID:Addgene_113240)

  • For your References section:

    Structures of the CRISPR genome integration complex. Wright AV, Liu JJ, Knott GJ, Doxzen KW, Nogales E, Doudna JA. Science. 2017 Sep 15;357(6356):1113-1118. doi: 10.1126/science.aao0679. Epub 2017 Jul 20. 10.1126/science.aao0679 PubMed 28729350
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