pPI304
(Plasmid #113232)

• Purpose: extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP
• Depositing Lab: Rob Kay
• Publication: Paschke et al PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018.

Backbone

• Vector backbone: pDM1209
• Backbone size w/o insert (bp): 9346
• Vector type: Dictyostelium discoideum expression vector, extrachromosomal, double reporter
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LifeAct & pkgE-PH
• Species: Synthetic
• Promoter: act15
• Tag / Fusion Protein:
  • mCherry & GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry