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pPI226
(Plasmid #113228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDM1501
  • Backbone size w/o insert (bp) 6205
  • Vector type
    safe haven knock-in plasmid for the act5 locus, LifeAct-mCherry
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LifeAct
  • Species
    Synthetic
  • Promoter act5
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPI226 was a gift from Rob Kay (Addgene plasmid # 113228 ; http://n2t.net/addgene:113228 ; RRID:Addgene_113228)

  • For your References section:

    Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM. PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018. 10.1371/journal.pone.0196809 PubMed 29847546
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