pPI180
(Plasmid
#113222)
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Purpose(Empty Backbone) generic knockin vector for C-terminal tagging with GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBlueScriptII
- Backbone size (bp) 5554
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Vector typegeneric knockin vector for C-terminal tagging with GFP
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPI180 was a gift from Rob Kay (Addgene plasmid # 113222 ; http://n2t.net/addgene:113222 ; RRID:Addgene_113222) -
For your References section:
Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM. PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018. 10.1371/journal.pone.0196809 PubMed 29847546
