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Addgene

pCS2-amo
(Plasmid #113190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2-MT
  • Vector type
    Mammalian Expression ; in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    amo
  • Alt name
    almost there
  • Species
    D. melanogaster (fly)
  • Mutation
    Y602H
  • GenBank ID
    AY437926.1
  • Entrez Gene
    Pkd2 (a.k.a. Dmel_CG6504, AMO, Amo, CG6504, Dmel\CG6504, Dmpkd2, PKD2, TRPP, TRPP2, amo, pkd2)
  • Promoter simian CMV IE94

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-amo was a gift from Craig Montell (Addgene plasmid # 113190 ; http://n2t.net/addgene:113190 ; RRID:Addgene_113190)

  • For your References section:

    A flagellar polycystin-2 homolog required for male fertility in Drosophila. Watnick TJ, Jin Y, Matunis E, Kernan MJ, Montell C. Curr Biol. 2003 Dec 16;13(24):2179-84. S0960-9822(03)00913-8 [pii] PubMed 14680634
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