MEI-S332-GFP genomic in pCaSpeR
(Plasmid
#113174)
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PurposeIn P element vector for transposon insertion into Drosophila genome
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaSpeR4
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Vector typeP insertion vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemei-S332 (Sgo)
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SpeciesD. melanogaster (fly)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MEI-S332-GFP genomic in pCaSpeR was a gift from Terry Orr-Weaver (Addgene plasmid # 113174 ; http://n2t.net/addgene:113174 ; RRID:Addgene_113174) -
For your References section:
Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions. Kerrebrock AW, Moore DP, Wu JS, Orr-Weaver TL. Cell. 1995 Oct 20;83(2):247-56. 0092-8674(95)90166-3 [pii] PubMed 7585942