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Addgene

MEI-S332-GFP genomic in pCaSpeR
(Plasmid #113174)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113174 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCaSpeR4
  • Vector type
    P insertion vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mei-S332 (Sgo)
  • Species
    D. melanogaster (fly)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MEI-S332-GFP genomic in pCaSpeR was a gift from Terry Orr-Weaver (Addgene plasmid # 113174 ; http://n2t.net/addgene:113174 ; RRID:Addgene_113174)
  • For your References section:

    Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions. Kerrebrock AW, Moore DP, Wu JS, Orr-Weaver TL. Cell. 1995 Oct 20;83(2):247-56. 0092-8674(95)90166-3 [pii] PubMed 7585942