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pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP
(Plasmid #113156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC407
  • Backbone manufacturer
    NIDA OTTC
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 6981
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Two gRNAs for rat Rosa26
  • gRNA/shRNA sequence
    GAGTCTTTCTGGAAGATAGG and GCAGATCACGAGGGAAGAAG
  • Species
    R. norvegicus (rat)
  • Promoter mU6 and hU6

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CCTCGCACAGACTTGTGGGA
  • 3′ sequencing primer AAATGGACTATCATATGCTTACCGTAACTTGAAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP was a gift from Brandon Harvey (Addgene plasmid # 113156 ; http://n2t.net/addgene:113156 ; RRID:Addgene_113156)

  • For your References section:

    Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. 10.1016/j.neuron.2019.01.035 PubMed 30792150
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