aL-mEos3.2
(Plasmid #113154)

• Purpose: constrained LFA-1-mEos3.2 fusion
• Depositing Lab: Timothy Springer
• Publication: Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. doi: 10.1016/j.celrep.2018.01.062.

Backbone

• Vector backbone: pcDNA3.1 hygro (-)
• Backbone size w/o insert (bp): 5596
• Total vector size (bp): 9708
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: full-length human aL with mEOS3.2 in betapropeller
• Alt name: aL-mEOS3.2
• Alt name: CD11a
• Alt name: integrin subunit alpha L
• Species: H. sapiens (human)
• Insert Size (bp): 4212
• Entrez Gene: ITGAL (a.k.a. CD11A, LFA-1, LFA1A)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEOS3.2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AflII (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry