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Chong Zhang E. coli Genome-wide Inhibition Library
(Pooled Libraries #113134, #113135, #113136, #113137, #113138, #113146, #113147)

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  • Purpose

    This genome-scale CRISPR inhibition library targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. Additionally, 2,400 gRNAs without any significant hit across the E. coli genome are provided as a negative control. The library is divided into 5 sublibraries defined by biological categories and includes a negative control (NC) control library and an additional custom test library targeting 86 genes (50 gRNAs per gene).

  • Vector Backbone

    pTargetF_lac_sacB-1-m - does not express dCas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 113134 Lib 1 - Essential, stress response, biosynthesis genes† 1 $275 Add to Cart
Pooled Library 113135 Lib 2 - Other genes† 1 $275 Add to Cart
Pooled Library 113136 Lib 3 - Transport & other enzymatic genes† 1 $275 Add to Cart
Pooled Library 113137 Lib 4 - DNA-binding, carbohydrate metabolic, catabolic, RNA-coding genes† 1 $275 Add to Cart
Pooled Library 113138 Lib 5 - Promoters, ribosome binding sites† 1 $275 Add to Cart
Pooled Library 113146 Lib NC - Non-targeting gRNAs (negative controls)† 1 $275 Add to Cart
Pooled Library 113147 Lib Test - Customized library targeting 86 genes† 1 $275 Add to Cart
Available to Academic and Nonprofits Only

  • † A dCas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with plasmids expressing dCas9 such as pdCas9-J23111 (#113149) or pdCas9-J23109 (#113151).

  • If you would like to receive Lib 1 - 4 + NC (113134 - 7 + 113146) in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted gRNAs
113134 810+ 11,008
113135 1665+ 21,438
113136 857+ 12,502
113137 815+ 10,723
113138 7286† 10,257
113146 N/A 2400
113147 86 3451

  • + Number of clusters targeted by at least one gRNA; clusters are one single gene or homologous genes with similar sequences.

  • † Promoters/RBSs

  • Species
    Escherichia coli (E. coli)

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli Genome-wide Inhibition Library 1 - Essential, stress response, biosynthesis genes was a gift from Chong Zhang (Addgene #113134)
    E. coli Genome-wide Inhibition Library 2 - Other genes was a gift from Chong Zhang (Addgene #113135)
    E. coli Genome-wide Inhibition Library 3 - Transport & other enzymatic genes was a gift from Chong Zhang (Addgene #113136)
    E. coli Genome-wide Inhibition Library 4 - DNA-binding, carbohydrate metabolic, catabolic, RNA-coding genes was a gift from Chong Zhang (Addgene #113137)
    E. coli Genome-wide Inhibition Library 5 - Promoters, ribosome binding sites was a gift from Chong Zhang (Addgene #113138)
    E. coli Genome-wide Inhibition Library NC - Non-targeting gRNAs (negative controls) was a gift from Chong Zhang (Addgene #113146)
    E. coli Genome-wide Inhibition Library Test - Customized library targeting 86 genes was a gift from Chong Zhang (Addgene #113147)

  • For your References section:

    For #113134, #113135, #113136, #113137, #113146: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH. Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x. PubMed 29946130
    For #113138, #113147: Improved sgRNA design in bacteria via genome-wide activity profiling. Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH. Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572. PubMed 29982721
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