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p3s-ABE7.10
(Plasmid #113128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 8574
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE 7.10
  • Alt name
    TadA-TadA*7.10-nCas9-NLS-HA
  • Species
    S. pyogenes Cas9, E.coli TadA
  • Insert Size (bp)
    5361
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (C terminal on insert)
    • HA epitope (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3s-ABE7.10 was a gift from Jin-Soo Kim (Addgene plasmid # 113128 ; http://n2t.net/addgene:113128 ; RRID:Addgene_113128)

  • For your References section:

    Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Ryu SM, Koo T, Kim K, Lim K, Baek G, Kim ST, Kim HS, Kim DE, Lee H, Chung E, Kim JS. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. 10.1038/nbt.4148 PubMed 29702637
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