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Addgene

EcRfaHKOW
(Plasmid #113125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    1C
  • Backbone manufacturer
    UC Berkeley MacroLab
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression, use E. coli BL21 (DE3) Codon+ RIL
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RfaH
  • Species
    Escherichia coli
  • Insert Size (bp)
    153
  • Mutation
    Deleted residues 1-107
  • Entrez Gene
    rfaH (a.k.a. b3842, ECK3834, JW3818, hlyT, sfrB)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP-N10-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EcRfaHKOW was a gift from James Berger (Addgene plasmid # 113125 ; http://n2t.net/addgene:113125 ; RRID:Addgene_113125)

  • For your References section:

    Mechanism for the Regulated Control of Bacterial Transcription Termination by a Universal Adaptor Protein. Lawson MR, Ma W, Bellecourt MJ, Artsimovitch I, Martin A, Landick R, Schulten K, Berger JM. Mol Cell. 2018 Sep 20;71(6):911-922.e4. doi: 10.1016/j.molcel.2018.07.014. Epub 2018 Aug 16. 10.1016/j.molcel.2018.07.014 PubMed 30122535
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