EcNusGFL
(Plasmid #113122)

• Purpose: Expresses full-length E. coli NusG
• Depositing Lab: James Berger
• Publication: Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4. doi: 10.1016/j.molcel.2018.07.014. Epub 2018 Aug 16.

Backbone

• Vector backbone: 1C
• Backbone manufacturer: UC Berkeley MacroLab
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 7000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For expression, use E. coli BL21 (DE3) Codon+ RIL.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NusG
• Species: Escherichia coli
• Insert Size (bp): 543
• Entrez Gene: nusG (a.k.a. b3982, ECK3973, JW3945)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-MBP-N10-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7-fwd
• 3′ sequencing primer: T7-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nathan Thomsen, Berger Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry