pBLind
(Plasmid
#113109)
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PurposeVector encoding the RFP gene under blue light-inducible promoter BindF1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbE8K
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Vector typeBacterial Expression ; Cell-free
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRFP
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SpeciesE. coli
- Promoter BindF1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBLind was a gift from Chueh Loo Poh (Addgene plasmid # 113109 ; http://n2t.net/addgene:113109 ; RRID:Addgene_113109) -
For your References section:
Cell-Free Optogenetic Gene Expression System. Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL. ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 10.1021/acssynbio.7b00422. Epub 2018 Apr 4. 10.1021/acssynbio.7b00422 PubMed 29596741