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PAK4-3'UTR-BS1
(Plasmid #113090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmirGLO Dual-Luciferase
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 7350
  • Total vector size (bp) 7853
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p21 Activated kinase 4
  • Alt name
    PAK4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    504
  • Mutation
    none
  • GenBank ID
    NM_001014834.2 NM_001014834.2
  • Entrez Gene
    PAK4
  • Promoter PGK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site DraI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer ACACGGTAAAACCATGAC
  • 3′ sequencing primer GTCCAAACTCATCAATGTA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAK4-3'UTR-BS1 was a gift from James Donahue (Addgene plasmid # 113090 ; http://n2t.net/addgene:113090 ; RRID:Addgene_113090)

  • For your References section:

    MiR-199a-3p decreases esophageal cancer cell proliferation by targeting p21 activated kinase 4. Phatak P, Burrows WM, Chesnick IE, Tulapurkar ME, Rao JN, Turner DJ, Hamburger AW, Wang JY, Donahue JM. Oncotarget. 2018 Jun 19;9(47):28391-28407. doi: 10.18632/oncotarget.25375. eCollection 2018 Jun 19. 10.18632/oncotarget.25375 PubMed 29983868
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