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Addgene

pEvolvR-enCas9-PolI5M
(Plasmid #113078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Total vector size (bp) 12546
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    enCas9-PolI5M

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains nonsense mutation in the chloramphenicol acetyltransferase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEvolvR-enCas9-PolI5M was a gift from John Dueber & David Schaffer (Addgene plasmid # 113078 ; http://n2t.net/addgene:113078 ; RRID:Addgene_113078)

  • For your References section:

    CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE. Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 10.1038/s41586-018-0384-8. 10.1038/s41586-018-0384-8 PubMed 30069054
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