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PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonecustom
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameenCas9-PolI3M-TBD
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains nonsense mutation in the chloramphenicol acetyltransferase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEvolvR-enCas9-PolI3M-TBD was a gift from John Dueber & David Schaffer (Addgene plasmid # 113077 ; http://n2t.net/addgene:113077 ; RRID:Addgene_113077) -
For your References section:
CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE. Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 10.1038/s41586-018-0384-8. 10.1038/s41586-018-0384-8 PubMed 30069054
Map uploaded by the depositor.
