DeltaNIRD KVS-1
(Plasmid
#113069)
-
PurposeExpresses worm truncated KVS1.2 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 113069 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCI-neo
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameK+ voltage sensitive channel subunit 1.2
-
SpeciesC. elegans (nematode)
-
Mutationfirst 40 AA deletion
-
Entrez Genekvs-1 (a.k.a. CELE_C53C9.3)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
DeltaNIRD KVS-1 was a gift from Federico Sesti (Addgene plasmid # 113069 ; http://n2t.net/addgene:113069 ; RRID:Addgene_113069) -
For your References section:
A new mode of regulation of N-type inactivation in a Caenorhabditis elegans voltage-gated potassium channel. Cai SQ, Sesti F. J Biol Chem. 2007 Jun 22;282(25):18597-601. doi: 10.1074/jbc.M702079200. Epub 2007 May 8. 10.1074/jbc.M702079200 PubMed 17488718