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Addgene

DeltaNIRD KVS-1
(Plasmid #113069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    K+ voltage sensitive channel subunit 1.2
  • Species
    C. elegans (nematode)
  • Mutation
    first 40 AA deletion
  • Entrez Gene
    kvs-1 (a.k.a. CELE_C53C9.3)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DeltaNIRD KVS-1 was a gift from Federico Sesti (Addgene plasmid # 113069 ; http://n2t.net/addgene:113069 ; RRID:Addgene_113069)

  • For your References section:

    A new mode of regulation of N-type inactivation in a Caenorhabditis elegans voltage-gated potassium channel. Cai SQ, Sesti F. J Biol Chem. 2007 Jun 22;282(25):18597-601. doi: 10.1074/jbc.M702079200. Epub 2007 May 8. 10.1074/jbc.M702079200 PubMed 17488718
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