DeltaNIRD C95S KVS-1
(Plasmid
#113068)
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PurposeExpresses worm truncated KVS1.2 C113S in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC113S K+ voltage sensitive channel subunit 1.2
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SpeciesC. elegans (nematode)
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MutationC113S+first 40 AA deletion
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Entrez Genekvs-1 (a.k.a. CELE_C53C9.3)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DeltaNIRD C95S KVS-1 was a gift from Federico Sesti (Addgene plasmid # 113068 ; http://n2t.net/addgene:113068 ; RRID:Addgene_113068) -
For your References section:
Oxidation of a potassium channel causes progressive sensory function loss during aging. Cai SQ, Sesti F. Nat Neurosci. 2009 May;12(5):611-7. doi: 10.1038/nn.2291. Epub 2009 Mar 29. 10.1038/nn.2291 PubMed 19330004