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Addgene

DeltaNIRD C95S KVS-1
(Plasmid #113068)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113068 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C113S K+ voltage sensitive channel subunit 1.2
  • Species
    C. elegans (nematode)
  • Mutation
    C113S+first 40 AA deletion
  • Entrez Gene
    kvs-1 (a.k.a. CELE_C53C9.3)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DeltaNIRD C95S KVS-1 was a gift from Federico Sesti (Addgene plasmid # 113068 ; http://n2t.net/addgene:113068 ; RRID:Addgene_113068)
  • For your References section:

    Oxidation of a potassium channel causes progressive sensory function loss during aging. Cai SQ, Sesti F. Nat Neurosci. 2009 May;12(5):611-7. doi: 10.1038/nn.2291. Epub 2009 Mar 29. 10.1038/nn.2291 PubMed 19330004